The 2-Minute Rule for flow cytometers
The 2-Minute Rule for flow cytometers
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Details-enriched characterization of cell populations. Morphological data from illustrations or photos can incorporate on the richness of flow cytometry info. For example, the figure demonstrates an or else typical apoptosis assay applying Annexin V and PI, adding mobile imaging to characterize cells in Each and every population to expose morphologically unique capabilities. These insights could not have been obtained from multiplex staining by yourself.
As samples are obtained about the Attune CytPix Flow Cytometer, the large-velocity brightfield digital camera captures and shops illustrations or photos of detected activities in a charge up to six,000 photographs per next, depending on the flow fee and picture sizing. For bigger overall flexibility, Attune Cytometric Computer software allows you to regulate the image capture frequency as necessary and select a certain gate for picture capture, if required.
Mix acoustic focusing technological know-how with higher-parameter Investigation for versatile spectral unmixing and conventional workflows.
The award-successful Invitrogen Attune CytPix Flow Cytometer brings together acoustic concentrating that has a substantial-speed brightfield camera for simultaneous significant throughput flow cytometry and superior resolution brightfield imaging.
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Additionally, it truly is illuminating to determine how many doublets slip by way of regular singlet gates, and excellent to validate the status of sudden double-optimistic functions in my Investigation."
Pair your Attune flow cytometer by having an autosampler for enhanced workflow effectiveness, and increase robotic integration for max throughput.
Reliable impression excellent even at substantial throughput Acoustic focusing along with a significant-velocity digicam Merge to graphic these T cells persistently at small or higher flow fees. Easily regulate concentration and digital camera settings to satisfy experimental needs.
Early indications of this contamination experienced to begin with been dismissed as particles. Backgating to discover new populations. Backgating imaged cells within the Attune CytPix Flow Cytometer also lets you use morphological features to discover exciting subpopulations that might not be clear from flow cytometry knowledge alone.
Customers can see celebration pictures in genuine-time to substantiate sample high-quality, optimize protocols, and document the morphology of each mobile inhabitants discovered for Evaluation. Imaging capture also Added benefits from acoustic concentrating, which helps you to situation the cells to make sure that a sharp, centered picture is obtained.
Sound waves enable exact mobile alignment, resulting in Extraordinary data good quality and ~10X speedier sample flow charges.
We Formerly shown the power of imaging Vehicle-T/Ramos cell interactions. Allow’s have a look at just the population of finest fascination, the double optimistic activities, to learn more. We can now use prolonged morphological parameters (in this article, circularity and skewness of depth) to additional study the options of such populations and refine gating on these gatherings, growing facts website robustness.
Leverage the strength of State-of-the-art technologies and innovation to accelerate your discoveries. Rely on while in the Attune Flow Cytometer family members to deliver the trustworthiness, speed, and precision that established your work aside.
Adaptable payment prepare from Agilent Economic Solutions permit you to obtain the essential instrumentation you will need for immediate job, or to develop your lab.
Gating on these extended parameters allows you to speedily and accurately determine populations of curiosity and confirm gating system with little or no guide assessment. The Attune program presents entry to condition-of-the-art machine learning Evaluation applications in just an expertly made user interface, rendering it exceptionally convenient to use.
Because the mobile line experienced Earlier been analyzed around the Attune CytPix Flow Cytometer, the scientists went back to the pictures and have been capable of doc the microbial infection a minimum of 5 days before.
Agilent Value Assure assures you at the very least 10 years of instrument use within the day you purchase. Usually, We'll credit score you With all the residual value of the method towards an upgraded design.
In this article, we show that by gating strategies using graphic-dependent quantitative parameters can distinguish interacting cells from coincident gatherings to additional properly evaluate interacting cells.